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For generation of standard curves for Escherichia coli and Pseudomonas aeruginosa bacteria as well as for resistance genes, plasmid constructs containing 247-bp fragment of bla tem , the 175-bp fragment of bla ctx , the 382-bp fragment of bla vim , the 163-bp fragment of sul1 and the 118-bp fragment of qnrS were used which is presented in SI Figs